Toxicological studies of newly developed nanomaterials of medical importance using in vivo and invitro model system.

Dr. Paramjit Grover Information Under Updation*

  • Project code : BT/PR/9998/NNT/28/84/2007
  • Designation : Scientist E-II
  • Department :Toxicology Unit - Biology Division
  • Affiliation : CSIR Indian Institute Of Chemical Technology, Hyderabad
  • Contact No. :91-40-27193135
  • Email Id
  • Sanctioned Amt :Rs. 92,12,064/-
  • Sanctioned Date :2007-11-01
  • Completion Date :2010-11-01
  • Web Url :
  • Project Status :Completed


Objectives of the Study

1. In the current project, we intend to generate the toxicological profile of nanomaterials (NM) of medical importance newly synthesized in our institute.
2. NM will be characterized as their physicochemical properties that may be important in understanding the toxic effects of the test materials include particle size and size distribution, agglomeration state, and porosity.
3. The nanomaterials would be Hydroxyapatite(HAP -10 and 20 nm) and Iron oxide (Fe2O3-10 and 20nm). For comparison, bulk materials of Hydroxyapatite and Iron oxide will be used. The Toxicity will be compared based on size and composition of nanomaterials.
4. The in vivo toxicity will be carried out in rats by oral/dermal/inhalation routes of exposure using OECD Guidelines.
5. Genotoxicity will be studied utilizing Micronucleus Test (MNT)/Comet Test in rats and invitro studies chromosome aberration(CA) test.
6. Toxicokinetic studies will be performed to investigate kinetics of nanomaterials using oral/dermal/inhalation routes of exposure.
7. The histopathology of rat organs will be investigated to examine the extent of tissue damage caused by nanomaterials after acute and toxicokinetic studies.
8. The mode of action of nanomaterials after oral/dermal/inhalation treatment will also be studied using biochemical and molecular end point related to oxidative stress and genotoxicity.
9. The in vitro toxicity will be evaluated with the rat liver cell line eg.BRL 3A.
10. The results originating from these studies will be useful in predicting the possible toxic hazards that may arise from the introduction of the nanomaterials into the environment. .

Proposed outcomes of the study

NM have unique properties that are highly desirable for application within the medical sector. The innovation properties of NM have already found their first commercial and emerging biomedical applictions. If the NM of the current study are found to be toxicologically safe two new of medical importance will emerge for the benefit of mankind.

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