Conjugation of engineered cytochrome P450 enzyme onto functionalized carbon nanotubes for bioelectrochemical degradation of pesticides and other pollutants.

Prof. Shyamlava Mazumdar Information Under Updation*

  • Project code : BT/PR9989/NNT/28/77/2007
  • Designation : Professor
  • Department :Department of Chemical Sciences
  • Affiliation : Tata Institute of Fundamental Research
  • Contact No. :022-22782363
  • Email Id :shyamal@tifr.res.in
  • Sanctioned Amt :Rs. 1,28,98,000/-
  • Sanctioned Date :2008-01-14
  • Completion Date :2013-01-14
  • Web Url : http://www.tifr.res.in/~shyamal/
  • Project Status :Completed

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Objectives of the Study

TIFR:

1. Design of highly stable mutant of cytochrome P450 enzyme that can recognize and catalyse degradation of different classes of halogenated hydrocarbons including pesticides. 

2. Development of the technique of immobilization of the chosen mutants of cytochrome P450 enzyme onto carbon nano-tubes to carry out electrochemically driven catalysis of the oxidation of the pollutants by the enzyme. Characterization of modified electrode by Atomic Force Microscopy. Quantification of kinetic parameters of the modified electrode of using scanning electrochemical microscopy and rotating disc electrodes. 

SPPU:
1. Design of micro arrays of cytochrome P450 bound carbon nano-tubes for making electrochemical devices to carry out oxidation of the pollutants.

Proposed outcomes of the study

Result from the present project would help in desinging a viable, stable bio-catalytic system that can efficiently remove pestisides and other toxic halogenated hydrocarbons that are of serious environmental concern in our country. The proposed studies would be lead to the understanding of the methods of tuning substrate specificity in the mono-oxygeneration reactions catalysed by cytochrome P450 enzymes and also help to design stable enzyme system with required biocatalytic properties. COnjugation of the enzyme with the carbon nanotubes would enable carrying out the catalytic reaction electrochemically and help in designing electrode arrays that can be used as regenrative, recyclabe heterogeneous biocatalysts.

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