Elicitor-loaded nanoparticles for crop protection.

Prof. Appa Rao Podile

  • Project code : BT/PR12859/NNT/28/437/2009
  • Designation : ProfessorVice-Chancellor, University of Hyderabad
  • Department :Department of Plant Sciences
  • Affiliation : University of Hyderabad
  • Contact No. :9100655777
  • Email Id :podilerao@gmail.com
  • Sanctioned Amt :Rs. 78,32,000/-
  • Sanctioned Date :2010-09-18
  • Completion Date :2014-09-17
  • Web Url : http://sls.uohyd.ac.in/faculty/ARP-CV-2018.pdf
  • Project Status :Completed


Project Abstract

HarpinPss, an elicitor from Pseudomonas syringae pv. syringae, induces systemic acquired resistance in non-host plants, thereby providing resistance to pathogen infection. We prepared non-adhesive chitosan nanoparticles (CSNPs) with high encapsulation efficiency. The harpinPss-loaded CSNPs (H-CSNPs) were round with good glomeration besides improving the stability of harpinPss and improving harpinPss bioavailability. H-CSNPs showed enhanced hypersensitive response and reduced severity of Rhizoctonia infection in tomato (Lycopersicon esculentum). Foliar spray application of H-CSNPs on tomato exhibited no visible HR however it has induced enhanced expression of defense related enzymes like POD and PAL which demonstrate the slow and steady release of harpinPss from the H-CSNPs. The transcripts for proteins involved in defense response were differentially expressed when treated with harpinPss, CSNPs and the H-CSNPs in tomato. Genes implicated in biosynthesis of jasmonic acid and aromatic amino acids were up-regulated, while the genes related to photosynthesis were down-regulated on H-CSNPs treatment. The use of CSNPs loaded with harpinPss would be effective for sustained release of harpinPss to induce resistance for a longer duration. We tracked the H-CSNPs entry into plant cells and harpinPss localized in chloroplasts.

Objectives of the Study

 To prepare, test and characterize elicitor-loaded nanoparticles for induction of broad-   spectrum disease resistance in tomato

Proposed outcomes of the study

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