Development of protocol for synthesis of nanoparticles metal tolerant microorganisms

Dr. Shilpanjali Sharma Information Under Verification By PI.*

  • Project code : BT/PR9013/NNT/28/17/2007
  • Designation :Group Leader
  • Department :Nanotechnology Capability, Governance and Developments Group
  • Affiliation :The Energy And Resources Institute (TERI), New Delhi, TDNBC, Haryana
  • Contact No. :91-11-24682100
  • Email Id :shilpas@teri.res
  • Sanctioned Amt :Rs. 48,23,032/-
  • Sanctioned Date :2007-08-01
  • Completion Date :2011-08-01
  • Web Url :

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Summary of The Proposed Outcome.

Nanotechnology is the study of phenomena exhibited by materials at the atomic and molecular level of dimensions ranging from a few nanometers to less thand hundred nanometers. The total global demand for nanscale materials, tools and devices was estimated at nearly $7.5 billion in 2003 and is expected to reach $28.7 billion in 2008, at an average annual growth rate of 30.6%. Although nanoparticle synthesis has been accomplished by chemical methods, there is an ever-growing need to develop clean, nontoxic and environmentally benign synthesis procedures. In this regard the use of microorganisms such as bacteria and fungi in the biosynthesis of metal nanoparticles and their applications holds immense potential. It is widely accepted that strains of microorganisms that can tolerate heavy metal stress may be potential biofactories for the synthesis of metal nanoparticles. At TERI, researchers have identified isolates of mycorrhizal fungi, other fungi and bacteria that are reported to tolerate and accumulate high levels of various heavy metals. The present proposal envisages the use of these efficient microbial strains for the synthesis of nanosized materials. The suitable isolates will be screened and subsequently tested for synthesis of nanoparticles and finally isolates specific processess will be developed for synthesis, detection and recovery of novel metal nanoparticles.


In this study, several microbial starins-bacteria, fungi and ectomycorrhiza were screened from an established genepool for their ability to synthesize Nickel and Chromium nanoparticles. Out of all the strains screened two starins S5 (Fungi) characterized as Aspergillus niger and E1 (Ectomycorrhiza) characterized as Paxilus involouts have potential to synthesize biomass concentration and pH revealed that Aspergillus niger strain S5 stable synthesis of Chromium nanoparticles occured at 3.5 pH value using 5gm of their biomass. Therefore Aspergillus niger strain S5, a good candidates for the intracellular systhesis of Chromium nanoparticles may be taken forward for large scale synthesis of these nanoparticles also especially since preliminary studies conducted in this project demonstrated that different techniques may be successfully used to break open the cells of strain S5 to release the nanoparticles.


1     Sarma, S., Swarnkar, M.K., channashettar, V., Das, M., Uppal, H.S., Adholeya, A., 2010 "Microorganisms as 'bio-factories' to synthesize industrially relevant nanoparticles. 2010, ,

2    Sawrnakar M. K., Veeranna Channashettar, Shilpanjali Sarma and Alok Adholeya Ectomycorrhizas: extending the capabilities of chromium-nanoparticles biosynthesis Mycorrhiza News 2009, 21, 34-39


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